Histological Examination of Callogenesis in Bisected Protocorm Culture of Pigeon Orchid (Dendrobium crumenatum Swartz)

Histological Examination of Callogenesis in Bisected Protocorm Culture of Pigeon Orchid (Dendrobium crumenatum Swartz)

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Callogenesis is a prerequisite step for the initiation of callus-mediated plant regeneration.Three-month-old protocorms of Dendrobium crumenatum Swartz (pigeon orchid) were bisected transversely and cultured on modified Vacin and Went (VW) solid medium containing 20 g L-1 sucrose, 2 g L-1 Phytagel added with NIGHT various concentrations of 1-naphthaleneacetic acid (NAA) (0, 0.1, 0.5 mg L-1) and 6-benzyladenine (BA) (0, 1, 2 mg L-1).The highest percentage of callus formation was obtained from the explant cultured on a modified VW supplemented with 0.

5 mg L-1 NAA and 1 mg L-1 BA.The callus originated from the subepidermal layer of the bisected protocorm and subsequently formed nodular structure after culture for 8 weeks.The callus mass contained small isodiametric cells with prominent nuclei Double Oven Glove and nucleoli within dense cytoplasm.